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NanoTemper Technologie

  1. NanoTemper tools are the quickest way to characterize proteins and perform protein sequence analysis for research in pharmaceutical, biotechnology and academia
  2. e Reactive) Company NanoTemper Technologies; Price Get Quote Get Quote for this Product; Catalog Number L001; Quantity Kit; Applications. Kit for 1h labeling and purification of 4x 100 µg protein with the dye NT-647 ; effective removal of free, unreacted dye ; achieve a 1:1 ratio of labeled protein to dye; the fluorescent dyes are.
  3. NanoTemper develops analytical instruments & technologies for protein analysis and characterization research in pharmaceutical, biotechnology and academia

Company NanoTemper Technologies; Price Get Quote Get Quote for this Product; Catalog Number MO-G007 / MO-G008 / MO -G009; Capacity 16 samples; Quantity EA; Applications Purification-free, label-free analysis of biomolecular interactions (Protein, Peptide, Small molecule, Nucleic Acids, Lipids), Glycobiology; Format Capillary Format; Sensitivity Microscale Thermophoresis can monitor the binding. Monolith NT.115 Getting Started About MST MicroScale Thermophoresis (MST) is an immobilization-free technology for measuring biomolecular interactions. The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in the molecular hydration shell, charge or size. Since one or all of these parameters changes with virtually every.

MicroScale Thermophoresis (MST) is an immobilization-free technology for measuring biomolecular interactions with a wide range of affinities (pM-mM).The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in the molecular hydration shell, charge or size NanoTemper Technologies GmbH. Floessergasse 4, 81369 München, Deutschland; www.nanotempertech.com; Filter. Jobs per E-Mail Ihre Suche ergab 1 Treffer Ergebnisse werden geladen... Datum Relevanz; 1 von 1; 1 von 1; IT-Administrator: Biotech goes Cloud (m/w/d) NanoTemper Technologies GmbH München Als Teil unseres internationalen Teams arbeitest Du in unseren IT-Projekten und bist für unsere. MicroScale Thermophoresis Purified RTA was labeled with amine reactive protein labeling kit RED-NHS Monolith (NanoTemper Technologies MO-L001) using red fluorescent dye NT- 647-NHS. 60µM of the dye was mixed in ratio 3:1 with 20µM of RTA in total volume of 200µL. Free dye was removed by buffer-exchange column chromatography into MST reaction buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM.

Monolith NT Protein Labeling kit RED-NHS from NanoTemper

  1. Microscale thermophoresis (MST) is a technology for the biophysical analysis of interactions between biomolecules.Microscale thermophoresis is based on the detection of a temperature-induced change in fluorescence of a target as a function of the concentration of a non-fluorescent ligand. The observed change in fluorescence is based on two distinct effects
  2. Instrumentation The measurements were done on a NanoTemper Monolith NT.015 instrument. All measurements were performed at 40 % LED and 40 % MST power, Laser-On time was 30 sec, Laser-Off time 5 sec. References Davey et al., Solvent mediated interactions in the structure of the nucleosome core particle at 1.9 a resolution. J.Mol.Biol. 2002 319: 1097-111 Verreault et al., Nucleosome Assembly by.
  3. NanoTemper Technologies is committed to the best customer experience and enabling researchers to easily, efficiently, and accurately characterize proteins. W..
  4. NanoTemper will replace any product that does not meet the specifications This warranty limits NanoTemper´s liability only to the cost of the Product and within the expiration date. No warranty is applicable unless all product components are stored in accordance with instructions NanoTemper assumes no responsibility or liability for any indirect or consequential loss or damage whatsoever. The.
  5. NanoTemper Technologies - Flößergasse 4, 81369 Munich, Germany - Rated 4.6 based on 7 Reviews If Google would start to develop biotechnology devices..

NanoTemper Analysis 1.2.20 software was used to fit the data and to determine the apparent K D values. The apparent dissociation constants were 37.9±1.0 μM and 23.3±0.6 μM for GAS and S+100, respectively (Figure 4). Substitution of A-to-G resulted in dramatic decrease in affinity of S+100 mut#1 (Figure 4), while mutant S+100 mut#2 showed no detectable binding thus confirming sequence. Ubiquitin is a versatile scaffold protein for the generation of molecules with de novo binding and advantageous drug-like properties. (labelling of SDF-1 according to NanoTemper L001 Monolith™ Protein Labeling Kit RED-NHS instructions). Incubation was carried out for 30 min on ice and for FACS analyzes (CyFLow ML, Partec) cells were washed twice with cold PBS. Fluorescence intensities of. Review date: 08 Feb 2017 | Monolith His-Tag Labeling Kit RED-tris-NTA The Monolith NT His-Tag Labeling Kit is designed for MicroScale Thermophoresis (MST) applications, the method of choice for easy, rapid, and precise characterization of binding affinities Protein-Protein Interaction Analysis in Different Buffer Systems Application Note NT-MO-012 Using MST to analyse the binding of the β-Lactamase TEM1 to BLIP Moran Jerabek-Willemsen1 2& Gideon Schreiber 1) NanoTemper Technologies GmbH, Munich, Germany 2) Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel Abstract The analysis of protein-protein.

NanoTemper - Quick & Accurate Protein Characterization

Ubiquitin is a versatile scaffold protein for the generation of molecules with de novo binding and advantageous drug-like properties. NanoTemper L001 Monolith™ Protein Labeling Kit RED-NHS. Norwegen Koenigreich-Anleihe (A1G5CF / NO0010646813): die Anleihe der Norwegen, Königreich hat eine Laufzeit bis 24.05.2023. Der jährliche Coupon/Zins beträgt 2,000%. Es handelt sich um eine. Sehen Sie sich das Profil von Nuska Tschammer auf LinkedIn an, dem weltweit größten beruflichen Netzwerk. 5 Jobs sind im Profil von Nuska Tschammer aufgelistet. Sehen Sie sich auf LinkedIn das vollständige Profil an. Erfahren Sie mehr über die Kontakte von Nuska Tschammer und über Jobs bei ähnlichen Unternehmen Catalog number (NanoTemper) each; Premium Capillaries: MO-K025: 2.00 € each capillary: Standard Cappillaries: MO-K022: 0.45 € each capillary: Protein Labeling Kit RED-NHS: MO-L001: 100.00 € each reactio Sarcodonin G Derivatives Exhibit Distinctive Effects on Neurite Outgrowth by Modulating NGF Signaling in PC12 Cells Chen-Yu Cao†, Cheng-Chen Zhang†, Xin-Wei Shi‡, Ding Li†, Wei Cao†, Xia Yin† and Jin-Ming Gao*,† † Shaanxi Key Labotory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, Chin

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Twenty micromolar of the purified human MDM2 protein (residues 1-118) was fluorescently labeled following NanoTemper's Protein Labeling Kit RED‐NHS protocol for N‐hydroxysuccinimide (NHS) (L001‐ NanoTemper Technologies, Munich, Germany), coupling NT647 dye to lysine residues. The labeled protein was pretested in standard treated, hydrophilic, hydrophobic, and premium‐coated MST. This is the first study to investigate that the Quercetin can directly bind with aurora B kinase and block aurora B kinase activity, resulting in the inhibition of lung cancer cells in vivo and in vi.. Briefly, purified Bcl2(Δ23) or Bcl-xL(Δ21) were fluorescently labeled using the NanoTemper protein-labeling kit RED (L001, NanoTemper Technologies, Munich, Germany). A constant concentration of 50 n m of either NT647-labeled Bcl2(Δ23) or NT647-labeled Bcl-xL(Δ21) was incubated for 20 min at 20 °C in the dark with different concentrations of VDAC (2 n m to 2 μ m ) in PBS containing 0.05%. aurora B kinase is highly expressed in several cancer cells and promotes tumorigenesis and progression, and therefore, it is an important target for drug to treat tumors. Quercetin was identified to be an antitumor agent. Herein, we report for the first time that quercetin inhibited aurora B activities by directly binding with aurora B in vitro and in vivo. Ex vivo studies showed that. Abstract aurora B kinase is highly expressed in several cancer cells and promotes tumorigenesis and progression, and therefore, it is an important target for drug to treat tumors. Quercetin was ide..

SilE labelling was performed using the Monolith NT Protein Labelling Kit RED-NHS according to the manufacturer's (NanoTemper, MO-L001) instructions which labels primary amines present on lysine residues as they are more readily accessible by solvents. The protein concentration was adjusted to 20 μM and 100 μl used for the labelling procedure. The excess dye was then washed out and the. Protein Labeling Kit RED (Cat#L001) (NanoTemper . Technologies GmbH, Germany) according to the supplied . labeling protocol. The aurora B protein were diluted in . a 20 mmol/L HEPES (pH 7.4) and 0. Proteins were fluorescently labeled with Alexa 647 according to the manufacture's protocol (L001, Nanotemper technologies, Germany). Labeled proteins were kept at 20 nM. The unlabeled protein was titrated starting at 50,000 nM and serially diluted in 1:1 ratio in reaction buffer (20 mM sodium phosphate pH 8, 150 mM NaCl, 2 mM EDTA, 5 mM DTT). The measurements were performed at 40% LED and 20. To perform the MST experiment, MtCRE1′ was labeled with NT‐647 dye using NanoTemper's Protein Labeling Kit RED (L001, NanoTemper Technologies, München, Germany). The labeled MtCRE1′ was kept at a constant concentration of 20 n m, and the MtHPt1 solution (initial concentration 500 μ m) was prepared as a dilution series of 15 concentrations. The analysis was carried out in a buffer.

labeled with the amine reactive Monolith Protein Labeling Kit Red-NHS (MO-L001, NanoTemper Technologies) by reacting a solution of a 3:1 dye:protein ratio for 30 min in the dark. Excess dye was removed using a 0.5 mL Zeba Spin Desalting Column with a 7-kD Measurement of FNR-NrdI Interaction by Microscale Thermophoresis (MST) Ingvild Gudim1, *, Marie Lofstad1, MO-L001 MonolithTM Protein Labeling Kit RED-NHS (Amine Reactive) (NanoTemper) (contains NT-647-NHS dye [store at -20 °C]; spin column for buffer exchange [store at 4 °C]; gravity flow column for purification [store at 4 °C]; labeling buffer [store at 4 °C]) 7. MO-K002 MonolithTM NT. Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis Article (PDF Available) in Scientific Reports 8(1. Recombinant cMCR-1 was labeled using the Monolith NT protein labeling kit RED (L001; NanoTemper Technologies, Munich, Germany) according to the manufacturer's protocol. cMCR-1 was adjusted to 200 nM with PBS buffer supplemented with 0.05% (v/v) Tween 20 Substrate analog interaction with MCR-1 offers insight into the rising threat of the plasmid-mediated transferable colistin resistance Article (PDF Available) in The FASEB Journal 32(2):fj.

  1. Four naphtho-γ-pyrones (fonsecinones A and C and aurasperones A and E) were identified as potential antibacterial agents against Escherichia coli, extended-spectrum β-lactamase (ESBL)-producing.
  2. MST analysis was performed using a NanoTemper Monolith NT.115 apparatus as described previously . Briefly, purified VDAC1 was fluorescently labeled using NanoTemper Protein labeling kit BLUE (L001, NanoTemper Technologies). A constant concentration of the protein was incubated with different concentrations of the tested inhibitor in PBS. Afterward, 3-5 μl of the samples were loaded into a.
  3. Microscale thermophoresis measurements were conducted on a NanoTemper Monolith NT.115 device (NanoTemper Technologies GmbH, München, Germany). Human proteasome β5i subunit (PSMB8, ProSpec, cat. no: ENZ-600) was transferred from the vendor buffer to 20 mM phosphate buffer (pH 8.0) with 10% glycerol and was labeled with RED fluorescent NT-647-NHS dye (NanoTemper, cat. no: L001) following the.

PDF | To date, few structural models of VHH antibody binding to low molecular weight haptens have been reported. Here, we report the crystal structure... | Find, read and cite all the research you. Differing isoforms of the cobalamin binding photoreceptor AerR oppositely regulate photosystem expression. Haruki Yamamoto, Mingxu Fang, Vladimira Dragnea, Carl E Bauer , Indiana University, United States; Research Article Oct 3, 2018. Cited 3; Views 576; Cite this article as: eLife 2018;7:e39028 doi: 10.7554/eLife.39028. Article; Figures and data; Jump to. Figures; Tables; Data availability. labeled by NT-647 using Mo-L001 MonolithNTMT protein label- ing kit RED-NHS (NanoTemper) following the manufacturer's instructions, and was added into the reaction system as ligand

MicroScale Thermophoresis (MST) Center for

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  1. PTEN globally suppresses growth in multiple cell types and inhibits neuronal axon growth and branching. Brosig et al. describe a mechanism for developing neurons to inhibit PTEN function by upregulating PRG2 in the axon. Although the overall growth capacity of neurons remains constant, PRG2 redirects growth to axon branches
  2. incubation at room temperature the samples.
  3. Inoyama et al. disclose the optimization of an indazole antitubercular targeting the β-ketoacyl-ACP synthase KasA. A structure-based approach has overcome significant issues with mouse metabolic stability and pharmacokinetics. A preclinical drug candidate has been delivered with efficacy in a mouse model of chronic M. tuberculosis infection at 5 mg/kg dosing

MST measurements were performed using a Monolith NT.115 instrument (NanoTemper). Capillaries were loaded into the instrument as sets of 16 point ligand titrations. Rb47b was labeled in PBS using the Monolith protein-labeling kit RED-NHS (amine reactive; MO-L001) according to the manufacturer's instructions. RRM1 to RRM3 were labeled using a His kit. Note that RRM2 was in Tris (25 m m Tris. To perform the MST experiment, MtCRE1′ was labeled with NT‐647 dye using NanoTemper's Protein Labeling Kit RED (L001, NanoTemper Technologies, München, Germany). The labeled MtCRE1′ was kept at a constant concentration of 20 n m , and the MtHPt1 solution (initial concentration 500 μ m ) was prepared as a dilution series of 15 concentrations. The analysis was carried out in a buffer. DLL4 (Life Technologies, cat. no. 10171-H02H) and JAG1 (R&D Systems, cat. no. 1277-JG) recombinant proteins were labelled using N-Hydroxysuccinimide (NHS)-ester chemistry according to the manufacturer's protocol (MO-L001 Monolith™ Protein Labeling Kit RED-NHS, NanoTemper Technologies, Munich, Germany). Binding interactions between recombinant Notch1-ECD (R&D Systems, cat. no. 3647-TK) or.

Application Notes - MST untangles the intricacy of a

Microscale thermophoresis - Wikipedi

  1. e Reactive) dye. Twenty microlitres titrations of non‐labelled ALIX‐V were mixed with 20‐μl labelled ALIX‐V in a constant concentration. Four microlitres were mounted on hydrophobic silicon capillary (K003 Monolith NT.115) and used for each measurement. From.
  2. e Reactive; #MO-L001; NanoTemper Technologies GmbH, Munich, Germany) was set to 100 nM, 20% LED and 20% MST laser power. Thereby, the fluorescence intensity measured for each fluorescent protein was comparable (100-150.
  3. Some bacteria are able to use a process called photosynthesis to convert energy from sunlight into another form of energy they can use to grow. Within the bacteria, structures known as photosystems are responsible for absorbing light and transferring the energy to other molecules. The levels of light surrounding the bacteria continually fluctuate

Application Notes - NanoTemper

Two pairs of new enantiomers with unusual 5,5-spiroketal cores, termed (±)-japonones A and B [(±)-1 and (±)-2], were obtained from Hypericum japonicum Thunb. The absolute configurations of. We are grateful to Dr Jörg Kudla for access to the NanoTemper station (J.K. acknowledges support from DFG (FOR964)) and to the staff at beamline BL44XU at SPring-8, Japan for their kind help. NT™ Protein Labeling Kit RED (Cat#L001) according to the supplied labeling protocol. Labeled synaptotagmin 1 C2AB was used at a concentration of ~ 40 nM. For the label-free synaptotagmin 1 C2AB was used at 1 µM. Calcium and magnesium chloride were titrated in 1:1 dilutions beginning at a final concentration of 10 mM

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Our data strongly suggest that dihydrostreptomycin binds to a specific site on MscL and modifies its conformation, (Cat#L001) according to the supplied protocol. Labeled MscL was kept constant at 50 nM, all samples tested were diluted in a 20 mM HEPES (pH 7.4) and 1 mM DDM. After a 10 min incubation at RT, the samples were loaded into Monolith standard-treated capillaries, and the. Eupafolin is the main bioactive component extracted from the traditional Chinese medicine Ay Tsao (Artemisia vulgaris L.), and its anti-tumor activity has had been studied in previous researches. T-LAK cell-originated protein kinase (TOPK) belongs to serine/threonine protein kinase and is highly expressed in several cancer cells and tissues, such as colon cancer, lung cancer, esophagus cancer. These findings revealed a strong increase in hippocampal and cortical synaptic plasticity when acutely interfering with Nogo-A/S1PR2 signaling, similar to previous results obtained by blocking Nogo-A. We thus provide a novel biological concept of multi-ligand GPCR signaling in which this sphingolipid-activated GPCR is also bound and activated by the high molecular weight membrane protein Nogo-A Samples of 20 μ m ZnT8cR or ZnT8cW were labelled with amine‐reactive Monolith NT.115 labelling kit Red‐NHS (#MO‐L001; NanoTemper Technologies) as per the manufacturer's instructions. Labelled proteins were diluted to a final concentration of 100 n m in 10 m m potassium phosphate, pH 8, 60 m m NaCl, 20 m m sucrose, 100 μ m TCEP, 1 m m EDTA, 0.05% (v/v) Tween‐20 Aspulvinone O, a natural inhibitor of GOT1 suppresses pancreatic ductal adenocarcinoma cells growth by interfering glutamine metabolism Weiguang Sun1†, Shanshan Luan1†, Changxing Qi1†, Qingyi Tong1, Shan Yan1, Hua Li1,2* and Yonghui Zhang1* Abstrac

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Protein Purification-free Method of Binding Affinity

Ubiquitin is a versatile scaffold protein for the

Video: Monolith His-Tag Labeling Kit RED-tris-NTA from NanoTemper

Near-native, site-specific and purification-free protein

Development of Recombinant Lactococcus lactis Displaying Albumin-Binding Domain Variants against Shiga Toxin 1 B Subunit. PLOS ONE, Dec 201 After 10-min incubation at room temperature the samples were loaded into Monolith™ standard-treated capillaries and the thermophoresis was measured at 25°C after 30-min incubation on a Monolith NT.115 instrument (NanoTemper Technologies, München, Germany). Laser power was set to 40% using 30 s on-time. The LED power was set to 100%. The equilibrium dissociation constan Microscale thermophoresis ligand binding measurements were performed using a Nanotemper Monolith NT.115 (Nano Temper technologies) as previously described [5960]. Briefly, recombinant Nogo-A-D20 was fluorescently labeled using the Amine Reactive Protein labeling kit RED (L001, Nano Temper technologies). The N-terminus and individual ECLs of S1PR2 were synthesized as peptides (JPT Peptide.

Targeting the N Terminus of eIF4AI for Inhibition of Its

NanoTemperTechnologies始创于2008年,总部位于德国慕尼黑,历经十余载发展,全球有超过150名员工,设立了13个分支机构,包括慕尼黑、北京、上海、旧金山、波士顿等。我们一直致力于为生命科学研究领域提供最优质的解决方案!作为集研发、生产用于生物分析创新型设备及软件的仪器制造商, The actin-binding capacity (Dissociation Constant, KD) of hrRNASET2 was measured using Monolith NT.115 (Nanotemper Technologies, Germany). Briefly, each sample was tested at serial 1:2 dilutions, from 10 μM to 0.31 μM hrRNASET2 in Buffer G containing ATP. Fluorescently labeled actin, prepared using the MO-L001 Monolith™ Protein Labeling Kit RED-NHS, was then added to each sample. The. This banner text can have markup.. web; books; video; audio; software; images; Toggle navigatio This invention relates to a method of determining ligands of macromolecules, said method comprising or consisting of (a) subjecting a sample comprising (i) complexes formed by said macromolecules and said ligands and (ii) unbound ligands to a method which separates said complexes from said unbound ligands; (b) releasing ligands from complexes obtained in step (a); and (c) subjecting the. https://doi.org/10.18632/oncotarget.16609 Mengzhu Zheng, Shanshan Luan, Suyu Gao, Li Cheng, Bin Hao, Jiacheng Li, Yao Chen, Xuemei Hou, Lixia Chen, Hua L

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H1 Receptors Feline immunodeficiency computer virus (FIV) induces opportunistic disease in chronically infected. Posted on December 21, 2019. Feline immunodeficiency computer virus (FIV) induces opportunistic disease in chronically infected felines, and both prednisolone and cyclosporine A (CsA) are clinically used to take care of complications such as for example lymphoma and stomatitis. Pre. NanoTemper Technologies GmbH, Floessergasse 4, Munich 81369, Germany n Bioquímica, Laboratorio de Investigacio Programa Institucional en Biomedicina cnico Molecular ENMyH-Instituto Polite Nacional, Guillermo Massieu Helguera No. 239, La Escalera Ticoman, D.F, Mexico City 07320, Mexico https://doi.org/10.18632/oncotarget.7984 Xiaoyu Zeng, Lin Liu, Mengzhu Zheng, Huimin Sun, Juanjuan Xiao, Tao Lu, Guangqian Huang, Pianpian Chen, Jianmin Zhang, Feng. Mangiferīna kā potenciāla glikokināzes aktivatora identificēšana, izmantojot uz struktūras balstītu virtuālo ligandu skrīning Aug 19, 2016 - and perceive changes in the concentration of specific molecules. (SNF) related and cAMP-regulated kinases from yeast and animals..SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding

(PDF) Ubiquitin is a versatile scaffold protein for the

sheet1 PO Number Begin Date End Date Supplier Line Desciption Purchase Type Justification Buyer Merchandise Amt 0000836765 CAROL KAKALEC KOHN Abstracts, manuscripts, publications and other articles for fellows and faculty to be edite 3 Material and Methods Assay conditions Binding of labeled anti-toxin antibody to SYD977 and SYD985 in PBST buffer: Protein Labeling: 7 µM murine IgG2a anti-toxin antibody was labeled in PBS buffer using the Protein Labeling Kit RED-NHS (L001, NanoTemper Technologies, Germany). Sample Preparation: The concentration of the labeled anti-toxin antibody was kept constant at 1.5 nM. For the.

A1G5CF Norwegen Koenigreich-Anleihe: 2,000% bis 24

NanoTemper Technologies 特约代理: 点击次数:1476 发布时间:2017-06-30 : 世界*实验材料供应商 NanoTemper Technologies 正式授权上海起发为其中国代理, NanoTemper Technologies 在一直是行业的标杆,一直为广大科研客户提供zui为的产品和服务,上海起发一直秉承为中国科研客户带来的产品,的服务,签约 NanoTemper. 上海起发实验试剂有限公司 版权所有 总访问量: 1927031 地址:上海浦东川沙镇川沙路6619号上海起发实验试剂有限公司 邮编:201203 电话:021-50724187 021-50724961 传真:021-50724961 手机:15921799099 联系人:杨经理 邮箱:fige007@163.com GoogleSitemap 网址:www.qfbio.com 技术支持:化工仪器网 管理登陆 ICP备案号.

This protocol describes how to measure protein-protein interactions by microscale thermophoresis (MST) using the MonolithTM NT.115 instrument (NanoTemper). We have used the protocol to determine the binding affinities between three different flavodoxin reductases (FNRs) and a flavodoxin-like protein, NrdI, from Bacillus cereus (Lofstad et al., 2016) Atechnology byNanoTemper. Microscale Thermophoresis isbased on aphysical principle, used for the. first time for biomolecular interaction studies. Itmeasures changes ofthe. NanoTemper assumes no responsibility or liability for any indirect or consequential loss or damage whatsoever. The above limited warranty is sole and exclusive. For information contact NanoTemper Technologies GmbH Flößergasse 4 81369 München Germany Tel.: +49 (0)89 4522895 0 info@nanotemper.de For ordering NanoTemper products: Phone: +49 (0)89 4522895 0 Fax: +49 (0)89 4522895 60 E-mail. Finally, the labelled proteins were dialysed with column B (Nanotemper L001) and eluted with 50?mM Tris-HCl (pH 8.0) supplemented with 0.02% Tween 20. For each assay, the labelled protein (about 1?μ?M) was incubated with the same volume unlabelled His-LURE1.2 of 12 different serial concentrations in 50?mM Tris-HCl (pH 8.0) supplemented with 0.02% Tween 20 at room temperature for 10?min. The. 6. MO-L001 Monolith TM Protein Labeling Kit RED -NHS (Amine Reactive) (NanoTemper) (contains NT-647-NHS dye [store at -20 °C]; spin column for buffer exchange [store at 4 °C]; gravity flow column for purification [store at 4 °C]; labeling buffer [store at 4 °C]) 7 For information contact NanoTemper Technologies GmbH Fl??ergasse 4 81369 München Germany Tel.: +49 (0)89 4522895 0 info@nanotemper.de For odering NanoTemper products: Phone: +49 (0)89 4522895 0 Fax: +49 (0)89 4522895 60 E-mail: order@nanotemper.de User Manual Monolith NT? Protein Labeling Kit V011 Date Feb 2012 6 www.nanotemper.de +49 (0)89 4522895 0 User Manual User Manual Monolith NT.

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